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AB Vector LLC ProGreen™ | 25uL
ProGreen™ baculovirus genomic vector DNA encodes Aequorea victoria green-fluorescent protein (GFP) Fig. 1. GFP allows monitoring of recombinant virus propagation, optimization of cultivation conditions, and titration of virus stocks by "terminal green dilution." The polyhedrin site is reserved for insertion of target genes under control of strong polyhedrin promoter. Initially, the polyhedrin site was occupied by E.coli β-galactosidase gene. To allow insertion of a target gene, β-galactosidase gene was removed from the baculovirus DNA using Bsu36.I restriction endonuclease which cleaves immediately downstream of the polyhedrin promoter, in the β-galactosidase gene and in the downstream essential gene ORF 1629.
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